Answer:
Step-by-step explanation:
Cell fractionation and light microscopy are two different techniques that can be used to study various organelles in cells, and they provide different types of information.
Cell fractionation is a technique that involves breaking open cells and separating the different cellular components based on their size, shape, and density. This allows researchers to isolate specific organelles, such as mitochondria, lysosomes, or nuclei, and study their structure, function, and biochemical properties in more detail. By analyzing the composition, morphology, and enzymatic activity of isolated organelles, researchers can gain insights into their roles in cellular metabolism, signaling, and gene expression.
Light microscopy, on the other hand, is a non-invasive technique that allows researchers to visualize cells and organelles in their native state, without disrupting their structure or function. By illuminating cells with a beam of light and using lenses to magnify the image, researchers can observe the morphology, location, and behavior of different organelles, as well as the interactions between them. Light microscopy can provide information about the size, shape, and dynamics of organelles, as well as their localization within cells and their response to different stimuli.
In summary, cell fractionation and light microscopy are complementary techniques that can be used to study various organelles in cells, and they provide different types of information. Cell fractionation allows researchers to isolate and analyze specific organelles in detail, while light microscopy allows them to visualize and study organelles in their natural context. Together, these techniques can provide a more comprehensive understanding of the structure, function, and regulation of cells and their organelles.