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Why are the ddNTPs fluorescently labeled?

1 Answer

1 vote
Answer :

Labeling either the DNA sequencing primers or the individual nucleotides with a radioactive or fluorescent tag enables this visualization. In automated sequencing, the ddNTPs are labeled with fluorescent dyes that are detected by a scanner. This makes it possible for all four reactions (dGTP, dATP, dCTP, and dTTP) to be run in one lane, so you can have huge numbers of reactions on one gel.

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