Question

In SEC, in what volume would you expect molecules that are much smaller than the fractionation range of the Sephadex SP to elute?A. ViB. VmC. VavD. VrE. Vo

Answer 1

In size exclusion chromatography (SEC), molecules that are much smaller than the fractionation range of the Sephadex SP would elute in the void volume (Vo) of the column.

Step-by-step explanation:

In size exclusion chromatography (SEC), molecules that are much smaller than the fractionation range of the Sephadex SP would elute in the void volume (Vo) of the column.

The void volume (Vo) is the volume in the column that is not occupied by the stationary phase (Sephadex SP) and is usually represented by the elution of a large molecule such as a protein.

This means that molecules smaller than the fractionation range of Sephadex SP would pass through the column without being significantly retained and would elute early in the chromatogram.