Question

The discovery of major histocompatibility complex (MHC) restriction helped explain the phenomenon of non-self MHC recognition in graft rejection. Transplanted tissues from donors bearing MHC molecules that differ from those of the recipient are reliably rejected. The rapid and potent cell-mediated response to the transplanted tissue results from the presence in any individual of large numbers of T cells that are specifically reactive to particular non-self or allogeneic MHC molecules. Early studies on T cell responses to allogeneic MHC molecules used what technique?

ELISA
PCR
IEF
FACS
MLR

Answer 1

The MLR technique was used in early studies to understand T cell response to allogeneic MHC molecules in the context of tissue transplantation.

Step-by-step explanation:

Early studies on T cell responses to allogeneic MHC molecules utilized the Mixed Lymphocyte Reaction (MLR) technique. This process involves mixing lymphocytes from two different individuals and observing the interaction, particularly the proliferation of T cells as a response to the non-self MHC molecules presented by the donor's cells.

During tissue transplantation, this response can be so strong that, unless the MHC molecules are closely matched, it will result in the rejection of the transplanted tissue or organ. Furthermore, even with a good MHC match, transplant recipients often need lifelong immunosuppressant medication to prevent rejection.