Question

After growth in tissue culture, the infected cells lyse and the virus may be harvested from

A) the liquid supernatant after centrifugation, the lysate.
B) the remainder.
C) the quantal layer.
D) the monolayer

Answer 1

The virus can be harvested after cell lysis from the liquid supernatant following centrifugation, a process that separates the virions from the denser cellular debris.

Step-by-step explanation:

After growth in tissue culture and the eventual lysis of infected cells, the virus may be harvested from the liquid supernatant after centrifugation, which is referred to as the lysate. Centrifugation separates the virus-infected cellular debris from the liquid containing the virions. During this process, the denser cellular material forms a pellet at the bottom of the centrifuge tube, while the lighter liquid containing the virus remains as a supernatant on top. Viruses can also be isolated from infected cells using filtration methods, where filters remove materials larger than the virions, allowing the virus particles to be collected in the filtrate.