Question

What technique was used to silence irisin in APP and Presenilin 1 mutants?

1) shFNDC
2) intracerebroventricular injection
3) lentiviral vector
4) electrophysiology of hippocampus

Answer 1

The silencing of irisin in APP and Presenilin 1 mutants was likely achieved with a lentiviral vector commonly used in RNA interference (RNAi) strategies for gene silencing.

Step-by-step explanation:

The technique used to silence irisin in APP and Presenilin 1 mutants is likely to be RNA interference (RNAi), as it is the current technique of choice for gene silencing in mammalian cells and for therapeutic purposes. While the specific method isn't listed explicitly in the given options, RNAi often employs small interfering RNA (siRNA), which can be delivered via various vectors. In the options provided, a lentiviral vector would be a plausible method for delivering siRNA to target cells in vivo. This answer is inferred from the provided literature and general knowledge about gene silencing strategies.