Final answer:
The replisome enzymes work on both the leading and lagging strands during DNA replication, with the leading strand synthesized continuously in the direction of the replication fork and the lagging strand synthesized in Okazaki fragments in the opposite direction.
Step-by-step explanation:
DNA polymerase reads the template strand in the 3' to 5' direction and adds nucleotides only in the 5' to 3' direction. While the leading strand is synthesized continuously in the direction of the replication fork, the lagging strand is synthesized in short segments known as Okazaki fragments, which occur in the direction away from the replication fork. The process involves other enzymes such as primase, which synthesizes a primer to initiate synthesis, and DNA ligase, which seals the DNA fragments on the lagging strand. The coordination and synthesis of both strands ensure that the entire DNA molecule is accurately replicated.