Final answer:
NAD+ is reduced to NADH during glycolysis, and large numbers of high-energy electrons can deplete the cell's NAD+.
Step-by-step explanation:
The electron carrier NAD+ plays a crucial role in glycolysis. During glycolysis, NAD+ is reduced to NADH through the transfer of high-energy electrons. This reduction reaction occurs twice in glycolysis, producing 2 molecules of NADH. NADH carries the high-energy electrons to stage III of cellular respiration to produce more ATP.
When large numbers of high-energy electrons are produced in a short time, the cell's NAD+ can become depleted. This can happen when glycolysis is occurring at a rapid rate or during intense exercise. The depletion of NAD+ can affect the cell's ability to produce ATP through cellular respiration, leading to a decrease in energy production.