Final answer:
When the last cells to be rubbed off are sufficiently spaced apart during culturing, it can lead to different outcomes depending on the type of cells. In primary cell cultures, it allows the cells to continue growing and form a single layer resembling the tissue they were derived from. In continuous cell cultures, the cells can grow in piles or lumps resembling small tumor growths.
Step-by-step explanation:
When the last cells to be rubbed off are sufficiently spaced apart during culturing, it means there is enough distance between the cells. This can have different outcomes depending on the type of cells and the purpose of the culture: