Final answer:
In the gluconeogenesis pathway, the enzymes hexokinase, phosphofructokinase, and pyruvate kinase from glycolysis are replaced by different enzymes to facilitate the reverse biochemical reactions required to synthesize glucose.
Step-by-step explanation:
The enzymes from the glycolysis pathway that are not used by the gluconeogenesis pathway are hexokinase, phosphofructokinase, and pyruvate kinase. During gluconeogenesis, these enzymes are bypassed through the action of the specific enzymes glucose-6-phosphatase, fructose-1,6-bisphosphatase, and pyruvate carboxylase (or carboxykinase) respectively.
The other enzymes like phosphoglucose isomerase, aldolase, triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase, and enolase are common to both pathways but operate in the reverse direction during gluconeogenesis.