Question

Which is a characteristic of e. coli dna polymerases?

O pol iii functions as a single polypeptide chain that can form phosphodiester bonds.
O pol i functions as a multimeric protein that participates in dna repair.
O pol iii functions as a holoenzyme that polymerizes dna with high processivity.
O pol i functions as a core enzyme that clamps around the dn

Answer 1

E. coli DNA Pol III is a holoenzyme with high processivity important for DNA synthesis, while Pol I is multifunctional, involved in DNA repair and removal of RNA primers.

Step-by-step explanation:

A characteristic of E. coli DNA polymerases is that Pol III functions as a holoenzyme that polymerizes DNA with high processivity. In other words, DNA Pol III is the enzyme required for DNA synthesis and operates with high efficiency and speed, adding nucleotides to a growing DNA strand without dissociating frequently. Pol I functions as a multifunctional protein that participates in DNA repair and also possesses a 5→3' exonuclease activity, which is important for the removal of RNA primers used during DNA replication. This exonuclease activity part of Pol I can be detached by mild protease treatment, leaving behind the Klenow fragment that retains the polymerization and proofreading activities.