Question

In figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the entire ampicillin-resistance (amp) gene?

A) 1.50 kbp
B) 3.00 kbp
C) 0.17 kbp
D) 0.25 kbp
E) 1.08 kbp

Answer 1

The smallest piece containing the entire ampicillin-resistance (amp) gene after digestion with the appropriate restriction enzyme is approximately 3.00 kbp.

Step-by-step explanation:

In figure 9.1, the smallest piece containing the entire ampicillin-resistance (amp) gene will be the fragment that has the ampicillin resistance gene intact.

This means that the fragment should not be cut by the restriction enzyme.

Based on the information provided, the ampicillin-resistance gene is located in the plasmid.

Therefore, after digestion with the appropriate restriction enzyme, the smallest piece containing the entire amp gene would be the plasmid itself, which is approximately 3.00 kbp in size (option B).