Question

Which method is considered a viable counting method for microbial enumeration?

A. Pour plate and Spread plate method (Plate count)
B. Streak plate and Loop dilution method
C. Agar diffusion and Agar overlay method
D. Colony PCR and Microscopic observation

Answer 1

The viable counting method for microbial enumeration is the Pour plate and Spread plate method (Plate count). These methods involve creating serial dilutions of the microbial culture and inoculating them onto solid agar. The option (A) is correct.

Step-by-step explanation:

The viable counting method for microbial enumeration is the Pour plate and Spread plate method (Plate count). These methods involve creating serial dilutions of the microbial culture and inoculating them onto solid agar.

In the pour plate method, the culture is mixed in warm agar and poured into a Petri dish, while in the spread plate method, the culture is spread onto solid agar using a sterile spreader. The resulting colonies are then counted to estimate the number of cells in the original sample. Therefore, option (A) is correct.