Final answer:
The RNA sequence made from the DNA template 5'-GATATCGAT-3' is 5'-AUCGAUAUC-3', with uracil replacing thymine.
Step-by-step explanation:
When RNA is made from this DNA template 5'-GATATCGAT-3', the sequence would be different because in RNA, thymine (T) is replaced with uracil (U), and the sequence is complementary to the DNA template. Thus, the RNA sequence would be 5'-CUAUAAGCU-3', reading from the 5' to 3' end as RNA polymerase would create it. This makes the correct answer A. 5'-AUCGAUAUC'-3', where thymine in the DNA is replaced by uracil in the RNA.