Question

A copy of the coding region of a gene is inserted downstream from a different, stronger promoter, and cells are transformed with the recombinant DNA. The most likely reason for the insertion is to________.

Answer 1

The most likely reason for inserting the coding region of a gene downstream from a different, stronger promoter is to increase the level of gene expression.

Step-by-step explanation:

The most likely reason for inserting the coding region of a gene downstream from a different, stronger promoter is to increase the level of gene expression. Promoters are regions of DNA that bind transcription factors to initiate transcription. By inserting the coding region downstream from a stronger promoter, the gene can be transcribed and translated more efficiently, resulting in higher protein production.

For example, if a gene normally produces a low level of protein, inserting it downstream from a stronger promoter can increase the level of protein expression. This can be helpful in situations where a higher amount of a specific protein is needed, such as in medical research or biotechnology applications.

Overall, the insertion of the coding region downstream from a stronger promoter enhances gene expression and allows for increased production of the encoded protein.