Final answer:
All pre-ganglionic neurons utilize acetylcholine as their neurotransmitter.
Step-by-step explanation:
The statement that all pre-ganglionic neurons utilize acetylcholine as their neurotransmitter is true. In the autonomic nervous system, both sympathetic and parasympathetic ganglionic neurons are activated by acetylcholine released from preganglionic fibers. These neurons have nicotinic receptors in their cell membranes, which are ligand-gated ion channels. When acetylcholine binds to the receptor, ion channels open, allowing positive ions to cross the membrane and depolarize the ganglia.