Question

What are the sequences of the forward primer and the reverse primer? Make sure that you indicate which primer is the forward one and which primer is the reverse one. To receive full credit you must label the 5' and 3' ends of both primers.

Answer 1

The forward primer sequence is ctagggttccgcatCtcaattgacatggac and the reverse primer sequence is gtccatgtcaattgaGatgcggaaccctag. Both primers anneal to opposite DNA strands.

Step-by-step explanation:

The forward primer sequence is ctagggttccgcatCtcaattgacatggac and the reverse primer sequence is gtccatgtcaattgaGatgcggaaccctag. In both primer sequences, the 5' end is labeled as 5' and the 3' end is labeled as 3'. The forward primer anneals to the bottom DNA strand, while the reverse primer anneals to the top DNA strand.