Question

The application called multiple strand displacement amplification is a powerful tool because?

1) it allows single-cell genomic sequencing that can analyze uncultivated microorganisms.
2) it provides data on the pathogenic nature of the microorganism.
3) it identifies the transcriptome of the microorganism.
4) it provides errorless sequencing results for that microorganism.

Answer 1

Multiple strand displacement amplification is a valuable tool in biology primarily for enabling single-cell genomic sequencing and analyzing uncultivated microorganisms. This technique facilitates the study of microorganisms, forensic identification, and medical diagnostics by allowing for the amplification of minimal amounts of DNA.

Step-by-step explanation:

Multiple Strand Displacement Amplification

The application called multiple strand displacement amplification (MDA) is a powerful tool because it allows for the amplification of DNA from a single cell. This is crucial for single-cell genomic sequencing, enabling researchers to analyze uncultivated microorganisms. Through this method, even a scarce amount of DNA can be multiplied to a point where it is sufficient for sequencing and extensive study. This kind of amplification is essential in fields like forensic science, where identifying individuals from minute biological samples is necessary, or in medical diagnostics, where detecting pathogens rapidly can be critical. While MDA is an impressive technology, it does not inherently provide data on the pathogenic nature of the microorganism, identify the transcriptome, or guarantee errorless sequencing results. However, it does contribute to a broader understanding and identification of genetic anomalies which can have far-reaching applications in health and medicine.