Question

What are the sequences of the forward primer and the reverse primer? Make sure that you indicate which primer is the forward one and which primer is the reverse one. To receive full credit you must label the 5' and 3' ends of both primers.

Answer 1

The forward primer sequence is 5'-ctagggttccgcatCtcaattgacatggac-3' and the reverse primer sequence is 5'-gtccatgtcaattgaGatgcggaaccctag-3'.

Step-by-step explanation:

The forward primer sequence is 5'-ctagggttccgcatCtcaattgacatggac-3' and the reverse primer sequence is 5'-gtccatgtcaattgaGatgcggaaccctag-3'. The forward primer anneals to the top DNA strand while the reverse primer anneals to the bottom DNA strand. Both primers are written in the 5' to 3' direction and they have complementary sequences and inverse chain direction to facilitate Watson-Crick pairings.