Question

A scientist amplifies a region of DNA using PCR conditions which increase the error rate of TAQ polymerase. In this case, the scientist is performing PCR-based _____ _____.

Answer 1

The scientist is performing error-prone PCR, which involves deliberately introducing mutations or errors into the amplified DNA sequence. This can be useful for studying mutations' effects on protein function or exploring genetic variability.

Step-by-step explanation:

The scientist is performing PCR-based error-prone PCR. Error-prone PCR is a technique used to deliberately introduce mutations or errors into the amplified DNA sequence. It is achieved by altering the PCR conditions, such as increasing the error rate of the Taq polymerase used in the reaction. This can be useful in certain research applications, such as studying the effects of specific mutations on protein function or exploring genetic variability in a population.