Question

In the GST pull-down experiment, the entire bacterial core and distal promoter were used as a radioactive probe pre-bound with isolated GST-alpha CTD subunit. The outcome was a very sharp decline in the pulled-down radioactivity. What competitor was used to obtain this effect?

A. Core promoter sequence

B. UP element

C. Upstream activating sequence I, II and III

D. Discriminator

Answer 1

The core promoter sequence was used as a competitor in the GST pull-down experiment to obtain the sharp decline in pulled-down radioactivity.

Step-by-step explanation:

In the GST pull-down experiment, a competitor known as the core promoter sequence was used to obtain the sharp decline in pulled-down radioactivity. The core promoter sequence is an important element in the promoter region that binds to the transcription machinery and initiates transcription of a gene. By using the core promoter sequence as a competitor, it effectively competed with the isolated GST-alpha CTD subunit for binding, resulting in a decrease in radioactivity in the pulled-down fraction.