Question

What biochemical structure is crucial to the chain termination method of sequencing?

a) RNA primer
b) DNA ligase
c) Restriction enzyme
d) Dideoxynucleotide

Answer 1

The biochemical structure crucial to the chain termination method of sequencing is Dideoxynucleotide (ddNTP).

Step-by-step explanation:

The biochemical structure crucial to the chain termination method of sequencing is Dideoxynucleotide (ddNTP). The chain termination method, also known as the Sanger sequencing method, uses ddNTPs that lack a free 3' OH group necessary for further extension of the DNA chain. By incorporating a small proportion of fluorescently labeled ddNTPs into the DNA synthesis reaction, the sequencing process generates DNA fragments of different sizes that can be visualized and analyzed.