Final answer:
DNA ligase is not involved in the formation of the replication fork, which is formed by helicase separating the DNA strands. Single-strand binding proteins prevent DNA helix reformation, and DNA primase synthesizes an RNA primer for DNA polymerase to use.
Step-by-step explanation:
In the formation of the replication fork, DNA ligase is not involved. The replication fork is formed when helicase separates the DNA strands at the origin of replication. Topoisomerase breaks and reforms DNA's phosphate backbone ahead of the replication fork, relieving the pressure. Single-strand binding proteins prevent the DNA helix from reforming, allowing for replication to occur. DNA primase synthesizes an RNA primer used by DNA polymerase to form a daughter strand.