Question

Which of the following techniques cannot be used to determine the minimum inhibitory concentration of an antimicrobial drug against a particular microbe?

a) Broth dilution method
b) Agar diffusion method
c) E-test
d) Gram staining

Answer 1

Gram staining cannot be used to determine the minimum inhibitory concentration of an antimicrobial drug against a particular microbe.

Step-by-step explanation:

The technique that cannot be used to determine the minimum inhibitory concentration of an antimicrobial drug against a particular microbe is D) Gram staining. Gram staining is a technique used to classify bacteria into Gram-positive or Gram-negative based on their cell wall properties, and it is not used to determine the minimum inhibitory concentration of antimicrobial drugs. The other techniques mentioned, such as the broth dilution method, agar diffusion method, and E-test, are all commonly used methods for determining MIC.