Question

Given the locations of the MlyI cleavage site and recognition sequence, MlyI is NOT a:

1) Type I restriction enzyme.
2) Type II restriction enzyme.
3) Type III restriction enzyme.
4) Type IV restriction enzyme.

Answer 1

MlyI, which has a specific recognition sequence and cleavage site is most likely a Type II restriction enzyme, as opposed to Type I, III, or IV which have different cleavage characteristics.

Step-by-step explanation:

The student is asking about the types of restriction endonucleases based on the cleavage site and recognition sequence, particularly about MlyI. In brief, Type II restriction enzymes are known for their specific recognition and cleavage sites in a manner such that their recognition sites are palindromic and they cleave DNA at or near these sites. MlyI, with a defined recognition sequence and cleavage site similar to the provided example of EcoRI, is most likely a Type II restriction enzyme. Type I and Type III enzymes cleave DNA away from their recognition sites, while Type IV target modified DNA, such as methylated or hydroxymethylated bases.