Final answer:
The efficiency of an enzyme is determined by substrate concentration, temperature, and enzyme concentration. Increasing substrate concentration initially increases the reaction rate, while increasing enzyme concentration always increases the rate of the reaction.
Step-by-step explanation:
The efficiency of an enzyme is determined by various factors including substrate concentration, temperature, and enzyme concentration. Substrate concentration affects the rate of an enzyme-catalyzed reaction. Initially, as substrate concentration increases, the reaction rate also increases. However, once the enzyme molecules become saturated with substrate, the reaction rate levels off. Temperature also affects enzyme efficiency. At low temperatures, the rate of the reaction increases, but at high temperatures, the protein denatures, resulting in a decrease in enzyme activity. Enzyme concentration always influences reaction rate, and an increase in enzyme concentration leads to an increase in the rate of the reaction.