Final answer:
During anaphase of mitosis, sister chromatids are separated from each other. This separation ensures each daughter cell receives a complete set of chromosomes.
Step-by-step explanation:
Anaphase is the stage of mitosis during which sister chromatids are separated from each other. The centromeres divide and the sister chromatids are pulled apart by the shortening of the spindle fibers. One sister chromatid moves to one pole of the cell, and the other sister chromatid moves to the opposite pole.
The significance of this separation is that it ensures that each daughter cell receives a complete set of chromosomes. This is essential for genetic stability and the proper division of genetic material during cell division.