Question

Which type of chromatography can use the ligand-binding properties of a protein to produce a nearly pure product in a single step?

Answer 1

Affinity chromatography can utilize the ligand-binding properties of a protein to produce a nearly pure product in a single step.

Step-by-step explanation:

The type of chromatography that can use the ligand-binding properties of a protein to produce a nearly pure product in a single step is affinity chromatography. This technique takes advantage of the specific interaction between a ligand and a protein, allowing the protein of interest to bind to a stationary phase with the ligand attached to it, while other proteins pass through. After the unwanted proteins are washed away, the desired protein can be eluted by adding excess ligand or changing the buffer conditions.