Question

Order the four steps of Koch's postulates as they would normally be applied?

1) Isolation and cultivation of the suspected pathogen
2) Inoculation of the suspected pathogen into a healthy host
3) Recovery of the suspected pathogen from the diseased host
4) Observation of the same disease symptoms in the newly infected host

Answer 1

The correct sequence for applying Koch's postulates to determine the causative agent of a disease starts with recovery of the suspected pathogen from the diseased host, followed by isolation and cultivation, inoculation into a healthy host, and ends with the observation of the same symptoms in the new host.

Step-by-step explanation:

Order of Koch's Postulates

The correct order of Koch's postulates as they would normally be applied is:

1. Recovery of the suspected pathogen from the diseased host.
2. Isolation and cultivation of the suspected pathogen.
3. Inoculation of the suspected pathogen into a healthy host.
4. Observation of the same disease symptoms in the newly infected host.

These steps are based on the scientific method of identifying a microorganism as the causative agent of a disease. Originally proposed by Robert Koch, these postulates aimed to establish a link between a specific pathogen and a disease. While they were instrumental for the discovery of many infectious diseases, modern understanding recognizes limitations and exceptions to these postulates, such as the inability to grow certain pathogens like viruses and prions in pure culture, or the presence of certain pathogens in healthy individuals