Question

What would the result be of not blocking properly on a Western Blot?

a. Nonspecific bands, a lot of noise.
b. Clear and specific band patterns.
c. Increased sensitivity in band detection.
d. Enhanced signal-to-noise ratio.

Answer 1

Not blocking properly on a Western Blot can result in nonspecific bands and a lot of noise.

Step-by-step explanation:

Not blocking properly on a Western Blot can result in nonspecific bands and a lot of noise (a). This occurs because the primary antibody and secondary antibody bind to multiple nonspecific sites instead of the specific protein of interest. As a result, the signal becomes less specific and the noise level increases.