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What was the purpose of HRP (HorseRadish Peroxidase) in the medical testing/ELISA lab?

a) Detection of antibodies
b) Labeling antibodies
c) Initiating a colorimetric reaction
d) Forming antibody/enzyme combination (conjugate)

1 Answer

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Final answer:

HorseRadish Peroxidase (HRP) is used in the medical testing/ELISA lab for labeling antibodies. Its purpose is to catalyze a colorimetric reaction, indicating the presence of a specific antigen.

Step-by-step explanation:

In the medical testing/ELISA lab, the purpose of HorseRadish Peroxidase (HRP) is to label antibodies. In ELISA, a primary antibody captures the antigen, and then a secondary antibody conjugated with HRP is added. HRP catalyzes the conversion of a colorless substrate into a colored product, indicating the presence of the specific antigen. This colorimetric reaction helps in detecting and quantifying the antigen of interest.

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User Daquon
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