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What are the three key advantages or proteins processed using the CFM method?

1 Answer

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Final answer:

The CFM method for processing proteins is beneficial due to its cost-effectiveness, ease of handling, inclusion of important drug metabolism enzymes, and compatibility with protein identification and quantification techniques like LC-MS/MS.

Step-by-step explanation:

The Cross-Flow Microfiltration (CFM) method is a process used for protein purification that boasts several key advantages when compared to other methods, especially in the context of drug metabolism studies and proteomic analysis. The three main advantages include:

  • Cost-effectiveness and ease of handling, which make the CFM method an economical and user-friendly option for laboratories and researchers.
  • The presence of major drug metabolism enzymes, such as cytochrome P450s (CYPs) and UDP-glucuronosyltransferases (UGTs), which are essential for pharmacological studies and understanding substance processing within the body.
  • The method's suitability for protein identification and quantification, which are facilitated by techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), aiding in the accurate analysis of proteins within a mixture.

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User Ehsavoie
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