Final answer:
In microbiology, tissue samples are collected using sterile swabs and proper aseptic technique. These samples are spread onto TSA plates for culturing, or prepared for microscopy, and then examined to identify different bacteria present in the tissues.
Step-by-step explanation:
Tissues for microbiology are typically collected using sterile swabs or collection instruments, depending on the source of the sample. For instance, one might collect a sample of oral mucosa by gently rubbing a sterile swab over the inside of the cheek, and then smear it onto a microscope slide. For culturing certain bacteria, such as Micrococcus luteus and Enterococcus faecalis, a sterile swab might be moistened with sterile distilled water, aseptically collecting the sample, which is then spread onto a Trypticase soy agar (TSA) plate. After collection, samples are properly prepared, such as by heat-fixation or wet mount, and then observed under a microscope to count the different types of bacterial cells, such as cocci and bacilli, present in the tissues.