Final answer:
tRNA splicing involves cutting and rejoining in separate reactions to remove introns and reconnect exons. Spliceosomes recognize specific sequences at the ends of introns and catalyze the splicing process. The resulting mRNA is continuous and ready for translation.
Step-by-step explanation:
tRNA splicing involves cutting and rejoining in separate reactions. Before a protein can be synthesized from a pre-mRNA, all introns, non-coding regions, must be removed and exons, coding regions, must be rejoined. This process is called splicing and it is carried out by complexes of proteins and RNA molecules called spliceosomes.
Spliceosomes recognize specific sequences at the 5' and 3' ends of the introns and they catalyze the cutting and rejoining of the pre-mRNA to produce a continuous mRNA with only exons.
The spliceosome first recognizes the 5' end of the intron and cuts it, releasing the intron as a lariat-shaped structure. Then, the spliceosome recognizes the 3' end of the intron and joins the adjacent exons together, creating a continuous mRNA transcript ready for translation.