Final answer:
During Metaphase II, sister chromatids attach to kinetochore microtubules emanating from opposite poles and align at the cell's equator, preparing for their separation in Anaphase II.
Step-by-step explanation:
During Metaphase II, pairs of sister chromatids become attached to kinetochore microtubules which emanate from opposite poles of the cell. The sister chromatids are maximally condensed and aligned at the equator of the cell, known as the metaphase plate. This stage is critical as it ensures that each new cell will receive one copy of each chromosome after the sister chromatids are pulled apart during Anaphase II by the kinetochore microtubules, moving toward opposite poles of the cell.