Question

Which of the following would be an appropriate temperature for the first step of PCR

A) 94
B) 65
C) 37
D) 72
E) 55

Answer 1

The first step of PCR, denaturation, is optimally performed at 94°C, which is the correct temperature to separate the double-stranded DNA.

Step-by-step explanation:

An appropriate temperature for the first step of PCR (Polymerase Chain Reaction) is s94°C. Thi step is known as the denaturation step, where the double-stranded DNA is heated to separate it into two single strands, allowing the primers to bind in the subsequent annealing step.

Each of these steps within a PCR cycle requires a specific temperature: denaturation at around 95°C, annealing of primers usually around 55°C, and extension by the polymerase at 68-72°C.