Question

Which of the following recombinant tools is NOT used in DNA fingerprinting? ______

A) reverse transcription
B) restriction enzyme digestion
C) gel electrophoresis
D) PCR
E) neither PCR nor gel electrophoresis is used.

Answer 1

The correct option is A) reverse transcription. Reverse transcription is NOT used in DNA fingerprinting; instead, restriction enzyme digestion, PCR, and gel electrophoresis are commonly used to analyze DNA.

Step-by-step explanation:

The recombinant tool that is NOT used in DNA fingerprinting is A) reverse transcription. DNA fingerprinting typically involves treating the DNA with specific restriction enzymes to cleave specific DNA sequences, followed by PCR (Polymerase Chain Reaction) to amplify the DNA, and then using gel electrophoresis to separate the resulting fragments by size and create a banding pattern for comparison. Reverse transcription, which involves converting RNA to DNA using reverse transcriptase, is not a step used in this process, which instead focuses on analyzing DNA directly.