Question

After measuring the absorbance vs. concentration (and working with the same spectrophotometer) you realized you had worked with a wrong wavelength max. Which of the following will get affected in the regression line for calibration curve?

a) Slope of the regression line
b) Intercept of the regression line
c) Neither the slope nor the intercept
d) Both the slope and the intercept

Answer 1

Both the slope and the intercept of the regression line will be affected.

Step-by-step explanation:

When you work with a wrong wavelength max in a calibration curve, both the slope and the intercept of the regression line will be affected.