Final answer:
Two reactions in the TCA cycle involve the removal of water: the conversion of citrate to isocitrate and the conversion of isocitrate to α-ketoglutarate.
Step-by-step explanation:
The reactions in the TCA cycle that involve the removal of water are:
Step 2: The conversion of citrate to isocitrate involves the removal of a water molecule and is catalyzed by the enzyme aconitase.
Step 3: The conversion of isocitrate to α-ketoglutarate also involves the removal of a water molecule and is catalyzed by the enzyme isocitrate dehydrogenase.
These reactions play crucial roles in generating energy and producing high-energy electrons in the TCA cycle.