Question

How does DNase Footprinting work?

a) It measures enzyme activity in the cell
b) It identifies specific DNA-protein binding sites
c) It analyzes the presence of RNA in the nucleus
d) It quantifies the number of nucleotides in a DNA sequence

Answer 1

DNase Footprinting is a technique used to identify specific DNA-protein binding sites.

Step-by-step explanation:

DNase Footprinting is a technique used to identify specific DNA-protein binding sites.

It involves treating DNA with a specific restriction endonuclease, separating the resulting fragments by size on an agarose gel, and visualizing specific fragments using complementary DNA probes.

This method allows scientists to determine where proteins bind to DNA and can be used to study gene regulation, transcription factor binding, and protein-DNA interactions.