Final answer:
The size of fragment that is most difficult to determine in banding patterns is when there are two nearly identical fragments that emerge. This is because when the fragments are nearly identical in size, it becomes challenging to distinguish between them and accurately estimate their individual sizes.
Step-by-step explanation:
The size of fragment that is most difficult to determine in banding patterns is when there are two nearly identical fragments that emerge.
This is because when the fragments are nearly identical in size, it becomes challenging to distinguish between them and accurately estimate their individual sizes.
For example, if two fragments of similar size are formed, it would be difficult to determine which fragment belongs to which band and assign an accurate size to each fragment.