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In a PCR reaction, what is the ideal target region for the primers to bind to?

a) Exons
b) Introns
c) tRNA
d) rRNA

1 Answer

1 vote

Final answer:

The ideal target region for primers in PCR is exons, as they contain the protein-coding sequences that will be used to produce a polypeptide.

Step-by-step explanation:

In a PCR (Polymerase Chain Reaction), the ideal target region for the primers to bind to is the exon, because exons are the protein-coding sequences in eukaryotic genes that are transcribed into RNA and then spliced together to form mature mRNA, which codes for a polypeptide. Introns, on the other hand, are non-coding sequences that are removed from the primary transcript mRNA before it is translated into protein. Therefore, the correct answer is a) Exons.

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