Question

DNA renaturation is the basis of many DNA analysis methods. Which of the following techniques is NOT based on DNA renaturation?

A. Polymerase Chain Reaction (PCR)
B. Southern blotting
C. Northern blotting
D. Fluorescence in situ hybridization (FISH)

Answer 1

Polymerase Chain Reaction (PCR) is the technique that is not based on DNA renaturation.

Step-by-step explanation:

DNA renaturation is the process of re-forming double-stranded DNA from single-stranded DNA fragments.

It is the basis of many DNA analysis methods such as Southern blotting and Northern blotting.

However, Polymerase Chain Reaction (PCR) is NOT based on DNA renaturation.

PCR is a technique that allows for rapid amplification of specific DNA sequences without the need for renaturation.