Question

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by ____.

A) Net charge
B) Molecular mass
C) Abundance
D) Isoelectric point

Answer 1

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by their isoelectric point (pI), which is the pH at which a protein has no net charge.

Step-by-step explanation:

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by Isoelectric point (pI), which is the pH at which a protein has no net charge. Two-dimensional gel electrophoresis combines the separation of proteins based on their pI and molecular mass to achieve greater resolution.