Question

In a Lineweaver-Burk plot of the dependence of enzyme velocity on substrate concentration, what is the value of the function at X=0 (i.e., the Y-intercept)?

Answer 1

The Y-intercept in a Lineweaver-Burk plot represents the value of the function at X=0 and indicates the maximum velocity of the enzyme-catalyzed reaction when the enzyme is saturated with substrate.

Step-by-step explanation:

The Y-intercept in a Lineweaver-Burk plot represents the value of the function at X=0.

In terms of enzyme velocity and substrate concentration, the Y-intercept represents the maximum velocity of the enzyme-catalyzed reaction when the substrate concentration is approaching infinity (i.e., when the enzyme is saturated with substrate). It can be determined by finding the point where the line representing the Lineweaver-Burk plot intersects the Y-axis.