Question

A gel separation of RNA yields aberrantly migrating bands and smears. What are two possible explanations for this observation?

1) Contamination of the gel or buffers used
2) Degradation of the RNA sample
3) Improper loading of the gel
4) Insufficient voltage applied during electrophoresis

Answer 1

Possible explanations for aberrantly migrating bands and smears in a gel separation of RNA are contamination of the gel or buffers used and degradation of the RNA sample.

Step-by-step explanation:

Two possible explanations for aberrantly migrating bands and smears in a gel separation of RNA are:

1. Contamination of the gel or buffers used: If the gel or buffers used during the experiment are contaminated with impurities, it can lead to abnormal migration patterns and smearing of the RNA bands.
2. Degradation of the RNA sample: If the RNA sample is not properly handled and protected from degradation, it can break down into smaller fragments, resulting in aberrant migration patterns and smearing on the gel.