Question

Cycle sequencing differs from traditional PCR in that it results in _______.

A: Longer DNA strands
B: DNA sequencing products
C: Higher PCR amplification
D: More primer annealing

Answer 1

Cycle sequencing differs from traditional PCR in that it results in DNA sequencing products.

Step-by-step explanation:

Cycle sequencing differs from traditional PCR in that it results in DNA sequencing products. In traditional PCR, the goal is to amplify a specific DNA sequence, while in cycle sequencing, the goal is to determine the sequence of a DNA fragment. During cycle sequencing, the DNA sample is divided into four tubes, each containing a primer, DNA polymerase, and all four nucleotides. Limited quantities of one of the four dideoxynucleotides are added to each tube, which results in the termination of DNA synthesis at different points. This allows for the generation of DNA sequencing products of different lengths.