Question

In Fluorescence Microscopy (and Related Fluorescence-Based Techniques), what is covalent linking of a small organic fluorophore to an antibody to produce a fluorescent antibody useful for?

Answer 1

Covalent linking of a small organic fluorophore to an antibody in fluorescence microscopy produces a fluorescent antibody that is useful for identifying specific molecules or structures in a cell.

Step-by-step explanation:

Covalent linking of a small organic fluorophore to an antibody in fluorescence microscopy is done to produce a fluorescent antibody.

This fluorescent antibody is useful for identifying specific molecules or structures in a cell. When the fluorophore is attached to the antibody and illuminated with light, it emits visible light of a characteristic color, allowing the target molecule or structure to be detected and studied.