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A light microscope image is formed by

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User Vtokmak
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Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single or multiple lenses to allow a magnified view of the sample
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User Pcsutar
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Darkfield microscopy is a mode in which direct light is prevented from passing through the objective aperture, but a hollow cone of light forms an apex in the plane of the specimen. The image is formed by light scattering from features of the object.
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User Yonsy Solis
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