Question

The final step in a sanger dna sequencing reaction is to run the dna fragments on a gel. what purpose does this serve?

Answer 1

The choices can be found elsewhere and as follows:

A) It adds ddNTP to the end of each DNA fragment.
B) It changes the length of the DNA fragments.
C) It separates DNA fragments based on their charge.
D) It separates DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size.

I think the correct answer is option D, It separates DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size. Hope this answers the question. Have a nice day.